NASC Stock Detail Page
NASC ID: N16367
Name: SALK_073650 homozygous
ABRC stock number: CS16367
Description: Confirmed line isolated from original SALK_073650 line; homozygous for the insertion; genomic PCR analyses confirmed that a single T-DNA resides within intron 11; sequence analyses of both border fragments located the T-DNA insertion at position 1792 without any base deletion; intron 11 fails to splice due to insertion of a T-DNA copy; lacks N-acetyl-glucosaminyltransferase-I activity and is unable to form complex N-glycans in the Golgi apparatus; fails to react with complex glycan antiserum on immunoblots ("non-stainer" ).
Donation Date: 2008-10-10
Donated by: | Westfälische Wilhelms-Universität Münster Antje Von Schaewen |
Donor Number: cgl1-T
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000, SALK_073650)
Segregation status: homozygous
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Longer root hairs compared to wild type. Phenotype curated by ABRC
References
Frank, J. et al. 2008. Comparative analyses of Arabidopsis complex glycan1 mutants and genetic interaction with staurosporin and temperature sensitive3a. Plant physiology 148 (3):1354-67 .PubMed ID: 18768906.